The Portfolio of Sam Hodge

During my Honours thesis I produce a number of trucation mutation of a recombinant bovine Insulin Like Binding Protien bIGFBP2 to people in the know. By chopping off the carboxyl end of the DNA sequence cloned into a plasmid and then expressing the gene in tissue culutre in monkey kidney cells. I was able to show that after a certain amount of deletion that the protien was no longer functioning by binding to IGF-II like is is suppoed to. The work was done in conjunction with the CRC for Tissue Growth and Repair in John C Wallace's lab at the University of Adelaide.

Duration

Start Date: 

January, 1994

End Date: 

November, 1994

Organisation

Role(s): 

Student

Protien Expression

Recombination DNA Manipulation

PCR Mutagenisis

Protien Purification

Supervisor(s): 

John Wallace

Goran Forsberg

Employer: 

Student

Client: 

University of Adelaide